Production of Functional Lectin inPichia pastorisDirected by Cloned cDNA fromAleuria aurantia
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چکیده
منابع مشابه
Expression of a functional asialoglycoprotein receptor through transfection of a cloned cDNA that encodes a macrophage lectin.
The Gal/GalNAc-specific lectin on rat peritoneal macrophages (macrophage asialoglycoprotein binding protein, M-ASGP-BP) is structurally similar to rat hepatic asialoglycoprotein-binding protein (ASGP-BP) or rat hepatic lectin (RHL) and is highly homologous with the major component of RHL, RHL-1 (Ii, M, Kurata, H., Itoh, N., Yamashina, I., and Kawasaki, T. (1990) J. Biol. Chem. 265, 11295-11298)...
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250 BioTechniques Vol. 34, No. 2 (2003) be expected from an amplificationbased strategy. To our knowledge, this is the first time that such analysis has been conducted to assess the representation of a genomic library. In summary, we have developed a quick, facile protocol for constructing libraries from relatively small amounts of DNA. Using our method, we have shown that a representative, ran...
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Background: For several years, mammalian cloning by splitting an early embryo or nuclear transfer into oocytes method has been successfully performed. Cloning is now also possible using adult somatic cells. Although it has now been 15 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning is lo...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2003
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.67.2277